bcaP
168
amino acid transporter, major transporter for isoleucine, valine and threonine, minor serine and asparagine transporter
locus
BSU_09460
Molecular weight
49.55 kDa
pI
9.73
function
uptake of branched-chain amino acids, threonine, serine, and asparagine
product
amino acid transporter
essential
no
synonyms
bcaP, yhdG
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0531 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,023,350 1,024,747
Phenotypes of a mutant
no phenotype for the single mutant, the triple ''[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP] [gene|8BB8BDA602CC26B0A9E8C22666F0EC539306CF39|brnQ] [gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]'' mutant is strongly impaired in the transport of isoleucine and valine at low concentrations [Pubmed|25645558]
resistant to growth inhibition by high concentrations of threonine [Pubmed|32743959,25645558]
resistant to 4-hydroxythreonine [pubmed|25777134]
The protein
Catalyzed reaction/ biological activity
high affinity uptake of isoleucine and valine [Pubmed|25645558], (symport with H+)
uptake of threonine [pubmed|32743959], (symport with H+)
uptake of serine [pubmed|32743959], (symport with H+)
uptake of asparagine [pubmed|38193659], (symport with H+)
uptake of diaminopropionic acid [pubmed|38470260], (symport with H+)
Protein family
[wiki|amino acid-polyamine-organocation (APC) superfamily] (according to UniProt)
Structure
[PDB|5OQT] (from Geobacillus kaustophilus, 60% identity) [pubmed|29416041]
[AF|O07576]
Paralogous protein(s)
[protein|7E180420EB053D9EF7993EF600A169C446F905F9|mtrA]
[wiki|Localization]
cell membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]
description
[pubmed|22383849]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
Biological materials
Mutant
GP4157 (Δ[gene|75A874F0C344E8404BE39C32EFF59CE562193640|braB]::''aphA3'', Δ[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''ermC''), available in [wiki|Jörg Stülke]'s lab
GP4484 (Δ[gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''ermC''), available in [wiki|Jörg Stülke]'s lab [pubmed|38470260]
MGNA-B479 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::''erm''), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1478 NBRP B. subtilis, Japan]
BKE09460 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09460 BGSC] and in [wiki|Fabian Commichau]'s and [wiki|Jörg Stülke]'s labs), [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
BKK09460 ([gene|210C83EBEBD3DE4946BC511E0249D0032A7CE3D0|bcaP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCCCCTTCAACTCCCGA, downstream forward: _UP4_TAACCTTTTGATAAAGAGAG
References
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Time of last update: 2024-12-22 07:51:40
Author of last update: Robert.warneke