dacC
168
Class C penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase
locus
BSU_18350
Molecular weight
52.72 kDa
pI
5.41
function
carboxypeptidase
product
penicillin-binding protein 4A, D-alanyl-D-alanine carboxypeptidase
essential
no
synonyms
dacC, pbp
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2027 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,998,340 1,999,815
The protein
Catalyzed reaction/ biological activity
cleavage ofD-Ala-D-Ala interpeptide bridges in peptidoglycan [Pubmed|19758330]
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
Protein family
peptidase S13 family (single member, according to UniProt)
Structure
[PDB|1W5D] [pubmed|17582436]
[PDB|2J9P] (complex with a peptidoglycan mimetic peptide) [pubmed|17582436]
[AF|P39844]
[wiki|Localization]
during vegetative growth: distinct foci and bands at cell periphery [pubmed|14731276]
secreted, may be anchored in the membrane via a C-terminal amphiphatic alpha helix [pubmed|9733705]
Expression and Regulation
Operons
genes
[gene|993A863ED80BA5970C9145A35D407834B322C9DC|galM]-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]
description
[Pubmed|9733705]
sigma factors
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [Pubmed|9733705], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|10482513], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-B075 (dacC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1074 NBRP B. subtilis, Japan]
BKE18350 ([gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC, downstream forward: _UP4_TAATAGCGGGAGACCTGTTT
BKK18350 ([gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAATTTCCCCGCCTCTC, downstream forward: _UP4_TAATAGCGGGAGACCTGTTT
GFP fusion
3102 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 ([gene|DE4857E8A228F145EFB9B7AD817585258344ED1F|amyE]::Pxyl-gfp-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]1-1473 spc) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
3104 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]::pSG5044 (cat Pxyl-gfpa-[gene|20A3120D2A45449745C9162EDCF386ED3163851B|dacC]1-615) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
References
Reviews
Original Publications
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Time of last update: 2024-12-31 00:21:48
Author of last update: Jstuelk