gcvPB
168
glycine decarboxylase (subunit 2)
locus
BSU_24550
Molecular weight
54.26 kDa
pI
5.28
function
glycine utilization
product
glycine decarboxylase (subunit 2)
essential
no
ec
1.4.4.2
synonyms
gcvPB, yqhK, gcvP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1003 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,545,410 2,546,876
The protein
Catalyzed reaction/ biological activity
glycine + H+ + N6-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N6-(S8-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO2 (according to UniProt)
Protein family
GcvP family (with [protein|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA], according to UniProt)
[wiki|Cofactors]
pyridoxal phosphate (according to UniProt)
Structure
[PDB|1WYT] (the [protein|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[protein|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB] complex from Thermus thermophilus, 56% identity) [pubmed|15791207]
[AF|P54377]
[wiki|Localization]
cytoplasm (according to UniProt)
Expression and Regulation
Operons
genes
[gene|F6F449170276055E6D60D89E9E17A152A82CA166|gcvT]-[gene|DB0618EBA12013842ED3972165D0276F438D8EC1|gcvPA]-[gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]
description
[Pubmed|15472076]
regulation
induced by glycine [Pubmed|15472076]
the [wiki|Gly-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
Gly-box: RNA switch, [pubmed|31992591], in [regulon|other_regulator:Gly-box|Gly-box]
Biological materials
Mutant
MGNA-C470 (yqhK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2468 NBRP B. subtilis, Japan]
BKE24550 ([gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT, downstream forward: _UP4_TAAATAAAAACAGCTGTCTA
BKK24550 ([gene|20175DF9FCF07C34A05C572B089C33D1E1FE6DF2|gcvPB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAAAAATAAGTGCCTGGT, downstream forward: _UP4_TAAATAAAAACAGCTGTCTA
References
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Time of last update: 2025-04-06 20:04:01
Author of last update: Jstuelk