lpdV
168
2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
locus
BSU_24060
Molecular weight
48.51 kDa
pI
4.89
function
utilization of branched-chain keto acids
product
2-oxoisovalerate dehydrogenase (E3 subunit, dihydrolipoamide dehydrogenase)
essential
no
ec
1.8.1.4
synonyms
lpdV, yqiV, bkd
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1249 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,500,104 2,501,528
Phenotypes of a mutant
a strain lacking the [gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB] operon and the [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des] gene exhibits strongly reduced membrane fluidity and eventual growth arrest [pubmed|35037270]
defective in [category|SW.4.1.4|Swarming] motility (prolonged period of immotility prior to [category|SW.4.1.4|Swarming]) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
(R)-N6-dihydrolipoyl-L-lysyl-[protein] + NAD+ --> (R)-N6-lipoyl-L-lysyl-[protein] + H+ + NADH (according to UniProt)
Protein family
class-I pyridine nucleotide-disulfide oxidoreductase family (with [protein|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD] and [protein|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL], according to UniProt)
[wiki|Cofactors]
FAD (according to UniProt)
Structure
[PDB|2YQU] (from ''Thermus thermophilus (hb8 mutant)'', 42% identity, 55% similarity)
[AF|P54533]
Paralogous protein(s)
[protein|2ECF05AEE1549861EB3AE62139F0E9DEE0B0F632|acoL], [protein|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]: activation, [Pubmed|10094682], in [regulon|protein:4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10094682], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|10094682], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Biological materials
Mutant
MGNA-C379 (yqiV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2377 NBRP B. subtilis, Japan]
BKE24060 ([gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTGACTCACTCCTGTC, downstream forward: _UP4_TAAAAGCATAAAGGAGGGGC
BKK24060 ([gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGGTGACTCACTCCTGTC, downstream forward: _UP4_TAAAAGCATAAAGGAGGGGC
References
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Time of last update: 2024-11-09 07:36:32
Author of last update: Jstuelk