rsmH
168
SAM-dependent 16S rRNA m4C1402 methyltransferase
locus
BSU_15140
Molecular weight
35.15 kDa
pI
6.03
function
fine-tuning of the ribosomal decoding center
product
Ribosomal RNA small subunit methyltransferase H
essential
no
ec
2.1.1.199
synonyms
mraW, yllC, ylxA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0275 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,580,622 1,581,557
The protein
Catalyzed reaction/ biological activity
cytidine1402 in 16S rRNA + S-adenosyl-L-methionine --> H+ + N4-methylcytidine1402 in 16S rRNA + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
Structure
[PDB|3TKA] (from ''Escherichia coli'', 46% identity) [pubmed|22561317]
[PDB|1N2X] (from ''Thermotoga maritima'', 41% identity, 70% similarity) [Pubmed|12824489]
[AF|Q07876]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|A7131ACFA0CF5C222237C76E502D4AAD1F9BA3E4|mraZ]-[gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]-[gene|79712F4CF884A660C71B45B3397AEFA5C0AFA683|ftsL]-[gene|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]
description
[Pubmed|8636036,8244929]
regulation
constitutively expressed [Pubmed|15758244]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-B130 (ylxA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1129 NBRP B. subtilis, Japan]
1A818 ( ''mraW''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A818&Search=1A818 BGSC]
BKE15140 ([gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTGGTCCCACC, downstream forward: _UP4_TAACGAAACGCGAACGCAAA
BKK15140 ([gene|1F894D6895CE13AA006CFE13A702E46C568F6E79|rsmH]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15140 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTGGTCCCACC, downstream forward: _UP4_TAACGAAACGCGAACGCAAA
References
Reviews
Original Publications
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Time of last update: 2024-12-07 03:23:08
Author of last update: Jstuelk