abnA
168
endo-1,5-alpha-L-arabinosidase
locus
BSU_28810
Molecular weight
34.13 kDa
pI
8.62
function
arabinan degradation
product
endo-1,5-alpha-L-arabinosidase
essential
no
ec
3.2.1.99
synonyms
abnA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3507 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,949,053 2,950,024
The protein
Catalyzed reaction/ biological activity
Endohydrolysis of (1->5)-alpha-arabinofuranosidic linkages in (1->5)-arabinans (according to UniProt)
Protein family
[wiki|glycosyl hydrolase 43 family] (according to UniProt)
Structure
[PDB|1UV4]
[AF|P94522]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]
description
[Pubmed|14973026]
regulation
repressed in the absence of arabinose ([protein|search|AraR]) [Pubmed|14973026]
regulatory mechanism
[protein|A567466894AE9DE7CDE7816615433A37532297B5|araR]: repression, [Pubmed|14973026], in [regulon|protein:A567466894AE9DE7CDE7816615433A37532297B5|araR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|14973026], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
Biological materials
Mutant
BKE28810 ([gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT, downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC
BKK28810 ([gene|1F85A1324B8694FB3731376AFE6FFB162C096586|abnA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTGTTCAACCTCCTT, downstream forward: _UP4_TGAATTAAAAAGCCGGGCTC
References
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Time of last update: 2024-10-11 15:17:54
Author of last update: Melvin.boenninger