pbpX
168
Class C penicillin-binding protein X
locus
BSU_16950
Molecular weight
43.70 kDa
pI
9.71
function
endopeptidase
product
penicillin-binding protein X
essential
no
synonyms
pbpX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1680 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,765,859 1,767,034
Phenotypes of a mutant
increased sensitivity to lysozyme [Pubmed|21856855]
inactivation of ''[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]'' strongly restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
The protein
Protein family
[wiki|beta-lactamase family] (according to UniProt)
Structure
[PDB|3TG9] (a PBP from B. halodurans, aa 90-375, 28% identity)
[AF|O31773]
[wiki|Localization]
extracellular (signal peptide) [Pubmed|18957862]
during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]
during sporulation: both asymmetric septa and the prespore [pubmed|15758244]
Expression and Regulation
Operons
genes
[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]
description
[Pubmed|14762009]
regulation
expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]
sigma factors
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
[protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX]: sigma factor, [Pubmed|14762009,15758244], in [regulon|protein:E77364F274A520FCCA9F5C9504E317B047BA29E6|sigX regulon]
[protein|D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV]: sigma factor, [Pubmed|21926231], in [regulon|protein:D04629D798E458AD538809BB2B150B7FA81D2C7E|sigV regulon]
Biological materials
Mutant
MGNA-B074 (pbpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1073 NBRP B. subtilis, Japan]
BKE16950 ([gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
BKK16950 ([gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTTTTTCCTCCCAA, downstream forward: _UP4_TGATTGGCGATCTTCTTTTG
GFP fusion
3107 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]::pSG5047 (cat Pxyl-gfpa-[gene|1F2062E59607706FE26A22876544F91B3B13179E|pbpX]1-426) [pubmed|14731276], available in [wiki|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]
References
Reviews
Original Publications
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Time of last update: 2024-10-11 12:51:45
Author of last update: Jstuelk