accC
168
acetyl-CoA carboxylase (biotin carboxylase subunit)
locus
BSU_24340
Molecular weight
49.41 kDa
pI
4.72
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (biotin carboxylase subunit)
essential
yes
ec
6.3.4.14
synonyms
accC, yqhX
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0439 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,530,354 2,531,706
Phenotypes of a mutant
essential [Pubmed|12682299]
specific point mutations affecting [protein|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC] facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate [pubmed|33481774]
enhanced colony wrinkling upon knockdown [pubmed|36069446]
additional information
[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|59A7B26F810D7FEB07C176A8B2ECF6B83803DE49|ecsA], [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI], and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA] [gene|CB50289535EA537F63BADD459BD11AA7759A6658|rasP] double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in [gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA], [gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB], [gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC], [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD] or downregulation of the [wiki|FapR regulon] due to specific mutations in [gene|FCDE900167AE36377979E0CF7BC33C7F351B95D3|fapR]) [pubmed|37017514]
The protein
Catalyzed reaction/ biological activity
ATP + hydrogencarbonate + N6-biotinyl-L-lysyl-[protein] --> ADP + H+ + N6-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
acetyl-CoA + ATP + hydrogencarbonate --> ADP + H+ + malonyl-CoA + phosphate (according to UniProt)
[wiki|Domains]
[wiki|ATP-grasp domain] (aa 120-318) (according to UniProt)
Biotin carboxylation domain (aa 1-447) (according to UniProt)
Structure
[PDB|1BNC] (the protein from ''E. coli'') [Pubmed|7915138]
[AF|P49787]
Paralogous protein(s)
[protein|5C8815965146BCD8ED238258B3F820AA17241165|yotE]
Expression and Regulation
Operons
genes
[gene|ADD1CE87C1C5BA44FFF5499EA5E155C4C53A1C22|accB]-[gene|1DFFEB155028A5C6B4B344769EF7DAAC14983322|accC]-[gene|FACE7176B1A1F354CF27FB30E46A1140FAFB02F0|rfaA ]
description
[Pubmed|7592499]
Biological materials
Expression vectors
GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
pGP1025, N-terminal Strep-tag, based on [wiki|pGP172], purification from ''E. coli'', available in [wiki|Jörg Stülke]'s lab
pGP3646 (N-terminal 6xHis-tag, purification from E. coli, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
FLAG-tag construct
GP1483 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
GFP fusion
GP1491 (spc, based on [wiki|pBP43]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-31 06:25:43
Author of last update: Jstuelk