ptb
168
phosphate butyryltransferase
locus
BSU_24090
Molecular weight
31.62 kDa
pI
7.94
function
utilization of branched-chain keto acids
product
phosphate butyryltransferase
essential
no
ec
2.3.1.19
synonyms
ptb, yqiS, bkd
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0280 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,503,765 2,504,664
The protein
Catalyzed reaction/ biological activity
butanoyl-CoA + phosphate --> butanoyl phosphate + CoA (according to UniProt)
Protein family
phosphate acetyltransferase and butyryltransferase family (with [protein|3E343C05EE6FF04D5A54066E19DC6576784E09E7|pta], according to UniProt)
Structure
[PDB|1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)
[AF|P54530]
Expression and Regulation
Operons
genes
[gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]-[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd]-[gene|8E08A0333E4EC89003DA3C1DD8DCD39CDDC8624C|buk]-[gene|1FD06CB6E81C920EFC656DBE2A13D68B1AA68872|lpdV]-[gene|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]-[gene|A024921961A786294199EA12E04456C890ED8D8C|bkdAB]-[gene|262C9FD20C7A70B6F1FEB57735FA800F38EAB25A|bkdB]
description
[Pubmed|12823818]
regulation
induced in the presence of isoleucine or valine ([protein|search|BkdR]) [Pubmed|10094682]
regulatory mechanism
[protein|4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR]: activation, [Pubmed|10094682], in [regulon|protein:4CEBD81F485DD0B660E297FFC34A0F5270652184|bkdR regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|10094682], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|10094682], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
Biological materials
Mutant
MGNA-C417 (yqiS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2415 NBRP B. subtilis, Japan]
BKE24090 ([gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTACCACCTTTCTT, downstream forward: _UP4_TACACACATTAGGAGGAACG
BKK24090 ([gene|1CFF7202AC6078B6BAD1253F77FD4FE227C046DB|ptb]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTACCACCTTTCTT, downstream forward: _UP4_TACACACATTAGGAGGAACG
References
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Time of last update: 2025-01-13 21:19:05
Author of last update: Melvin.boenninger