ssbB
168
single-strand DNA-binding protein required to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU_36310
Molecular weight
12.35 kDa
pI
6.88
function
required for efficient genetic transformation
product
single-strand DNA-binding protein
essential
no
synonyms
ssbB, ywpH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0629 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,740,206 3,740,547
The protein
Catalyzed reaction/ biological activity
binds and protects internalized foreign ssDNA [Pubmed|22373918]
RecA-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
[wiki|Domains]
SSB domain (aa 1-104) (according to UniProt)
Structure
[PDB|3VDY] (in complex with ssDNA) [Pubmed|22373918]
[AF|C0SPB6]
Modification
phosphorylated on Arg-55 [Pubmed|22517742]
phosphorylated on Tyr by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|ptkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|ptpZ] [Pubmed|16549871]
Effectors of protein activity
phosphorylation strongly increases DNA-binding activity [Pubmed|16549871]
Paralogous protein(s)
[protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]
[wiki|Localization]
localizes to cell poles in competent cells [Pubmed|16009133]
localizes to the DNA entry pole during transformation [Pubmed|22373918]
transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]
Expression and Regulation
Operons
genes
[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]
description
[Pubmed|22383849]
regulation
expressed at the onset of stationary phase [Pubmed|14762004]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|11948146], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
genes
[gene|5BB6CE1D206F2BC278FD2F23CDAAE51CDAFFA2B6|ywpG]-[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]
description
[Pubmed|22383849]
Biological materials
Mutant
GP3522 (Δ[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]::kan), available in [wiki|Jörg Stülke]'s lab
MGNA-A591 (ywpH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/591 NBRP B. subtilis, Japan]
BKE36310 ([gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
BKK36310 ([gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
Expression vectors
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
References
Reviews
Original Publications
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Time of last update: 2024-12-22 09:32:22
Author of last update: Jstuelk