kbl
168
2-amino-3-ketobutyrate CoA ligase
locus
BSU_17000
Molecular weight
43.12 kDa
pI
6.25
function
threonine utilization
product
2-amino-3-ketobutyrate CoA ligase
essential
no
ec
2.3.1.29
synonyms
kbl
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0156 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,771,517 1,772,695
The protein
Catalyzed reaction/ biological activity
H+ + L-alanine + pimeloyl-[ACP] --> 8-amino-7-oxononanoate + CO2 + holo-[ACP] (according to UniProt)
Protein family
[wiki|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)
[wiki|Cofactors]
PLP (according to UniProt)
Structure
[PDB|1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) [Pubmed|11318637]
[AF|O31777]
Paralogous protein(s)
[protein|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|bioF]
Expression and Regulation
Operons
genes
[gene|8E64B62F15B92A2AACD68B913909100AE276D114|tdh]-[gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]
description
[Pubmed|22383849]
Biological materials
Mutant
BKE17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC
BKK17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC
References
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Time of last update: 2024-10-02 20:06:28
Author of last update: Melvin.boenninger