ddcP
168
DNA damage checkpoint recovery protease, promotes DNA damage checkpoint recovery
locus
BSU_15050
Molecular weight
38.61 kDa
pI
5.85
function
degradation of [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA]
product
DNA damage checkpoint recovery protease
essential
no
synonyms
ddcP, ylbL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3480 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,572,765 1,573,790
Phenotypes of a mutant
deletion of [protein|5ED8301B9681FDD5171A9344A971E891493A6558|ctpA] and [protein|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP] leads to accumulation of the checkpoint protein [protein|7BF591DCDC9635C605D76135481A8A9DB63EE861|yneA] [pubmed|29979679]
sensitivity to DNA damage [pubmed|29979679]
The protein
Protein family
Peptidase S16 family (with [protein|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA] and [protein|CA90AAE5320C20F93F5ED0F167294C28495756FF|lonB], according to UniProt)
[wiki|Domains]
TM helix (aa 9 - 29) [pubmed|29979679]
[wiki|PDZ domain] (aa 100 - 186) [pubmed|29979679]
Lon protease domain (aa 187 - 336) [pubmed|29979679]
Structure
[PDB|2KJP] (PDZ domain)
[AF|O34470]
[wiki|Localization]
cell membrane, with extracellular protease domain [pubmed|30315724]
Expression and Regulation
Operons
genes
[gene|DE3BCF20DE5BB438ACBB36B412817F6124CFDFFC|ylbK]-[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
description
[pubmed|22383849]
genes
[gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B249 (ylbL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1248 NBRP B. subtilis, Japan]
BKE15050 ([gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG
BKK15050 ([gene|1A5C1BDE844AA56B90C0E4A02D74978676487E99|ddcP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GCTAAAATGTTTTTTACGTA, downstream forward: _UP4_CTGAAAGCGAAAAGCACCTG
References
Reviews
Original Publications
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Time of last update: 2024-12-30 11:41:38
Author of last update: Jstuelk