glsA

glsA
168

glutaminase, low affinity for glutamine

locus
BSU_02430
Molecular weight
36.03 kDa
pI
5.32
Protein length
Gene length
function
glutamine degradation
product
glutaminase
essential
no
ec
3.5.1.2
synonyms
glsA, ybgJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2066 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
264,191  265,174
The protein
Catalyzed reaction/ biological activity
H2O + L-glutamine --> L-glutamate + NH4+ (according to UniProt)
Protein family
glutaminase family (with [protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM], according to UniProt)
Structure
[PDB|3BRM] [PDB|1MKI][Pubmed|18459799]
[AF|O31465]
Paralogous protein(s)
[protein|F31DD310F0D80ECC7C6D1E5DDB8EAF32565A3D51|ylaM]
Expression and Regulation
Operons
genes
[gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]-[gene|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT]
description
[Pubmed|15995196]
regulation
induced in the presence of glutamine ([protein|search|GlnL]) [Pubmed|15995196]
regulatory mechanism
[protein|4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL]: activation, [Pubmed|15995196], in [regulon|protein:4E6EA2EABBDAA427E8F9EE1AE1800AF8657CF18B|glnL regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15995196], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]→[gene|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT]

2024-11-07 02:38:13

ghost

65

1abc9708789b9480434727fa47a0c483a7d39864

AA0FCFADA92CADCC16FD2BF6A3187BADD37324D7

Biological materials
Mutant
GP4253 (Δ[gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::''cat''), available in [wiki|Jörg Stülke]'s lab
MGNA-B943 (ybgJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1942 NBRP B. subtilis, Japan]
BKE02430 ([gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE02430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG
BKK02430 ([gene|1A415DC85354373EA4866733C3AE43F510BD3C56|glsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK02430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCGAATTCCTCCTCACTG,  downstream forward: _UP4_TAAACATGAAAAAAGAGGTG
References
15995196,18459799

1A415DC85354373EA4866733C3AE43F510BD3C56

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Time of last update: 2024-11-08 11:27:43

Author of last update: MBenda