comGC
168
DNA uptake, major component of the pilus
locus
BSU_24710
Molecular weight
10.71 kDa
pI
6.32
function
genetic competence
product
major pilin
essential
no
synonyms
comGC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4537 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,557,673 2,557,969
Phenotypes of a mutant
knockout has complete loss of transformation competence [Pubmed|1968455]
The protein
Catalyzed reaction/ biological activity
captures extracellular DNA, pulls the bound DNA through the gap in the cell wall created during pilus biogenesis [pubmed|37695859]
Protein family
comGC family (single member, according to UniProt)
Structure
[AF|P25955]
[wiki|Localization]
cell surface [Pubmed|9723928]
localizes to the cell membrane and the cytoplasm [Pubmed|21278288]
localizes to one single cell pole in the majority of the cells [Pubmed|21278288]
localizes along the long axis of competent cells [pubmed|37292776]
Expression and Regulation
Operons
genes
[gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG]-[gene|8F3751A3D0910C9BED7FBCE90AFF7FDACFDCBC0B|spoIIIL]
description
[Pubmed|26735940,2507524]
regulation
expressed early during sporulation in the forespore ([protein|search|SigF]) [Pubmed|26735940]
regulatory mechanism
[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: activation, [Pubmed|8196543], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507524], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26735940], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
additional information
the ''[wiki|comGA]-[wiki|comGB]-[wiki|comGC]-[wiki|comGD]-[wiki|comGE]-[wiki|comGF]-[wiki|comGG]-[protein|search|spoIIIL]'' operon requires [wiki|NusA] for expression [http://www.nature.com/articles/nmicrobiol20157 Reference]
Biological materials
Mutant
BKE24710 ([gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT, downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
BKK24710 ([gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT, downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
BP914 (([gene|41DFEF8A6BC7E34A6681C26D92F2B08DC120A957|comGA]-[gene|EA1C841FC77A1994A85E2E0C0A554482C3EC9323|comGB]-[gene|19D76B80386F825109C5FB4A34881D5D20F366DB|comGC]-[gene|D323AEF5AFB4FD2A074ABAB76A39BE26A4C6436F|comGD]-[gene|FF4DB7FDCEB440064E11E65CB0DBFB93FC785CF0|comGE]-[gene|DD266DC0D78B5C5E82EED7CC7F55D0CF8FB4BF7A|comGF]-[gene|89442BFDFBFF264D0C7F12DEA6F8130C55D1F3F8|comGG])::phleo), available in [wiki|Fabian Commichau]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-12-30 22:21:21
Author of last update: Jstuelk