lipM
168
octanoyltransferase
locus
BSU_24530
Molecular weight
32.09 kDa
pI
5.93
function
biosynthesis of lipoic acid
product
octanoyltransferase
essential
no
ec
2.3.1.181
synonyms
lipM, yqhM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0095 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,543,968 2,544,804
The protein
Catalyzed reaction/ biological activity
transfers the octanoyl moiety from octanoyl-acyl carrier protein to the lipoyl domains of the 2-oxoacid dehydrogenases via a thioester-linked octanoyl-LipM intermediate [Pubmed|20882995]
L-lysyl-[protein] + octanoyl-[ACP] --> H+ + holo-[ACP] + N6-octanoyl-L-lysyl-[protein] (according to UniProt)
Protein family
octanoyltransferase LipM family (single member, according to UniProt)
[wiki|Domains]
[wiki|BPL/LPL catalytic domain] (aa 33-248) (according to UniProt)
Structure
[PDB|3A7A] (from ''E. coli'', 25% identity) [Pubmed|20089862]
[AF|P54511]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]-[gene|FF34DC303E8A7C9FAA205A6C2924961963B14EB1|mntR]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-C469 (yqhM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2467 NBRP B. subtilis, Japan]
BKE24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
BKK24530 ([gene|19CEB80CF637F1FED7C493EF743B4BFA632C8D44|lipM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK24530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTAAGACCTTCCTTAC, downstream forward: _UP4_TAAACAGGGCTTTTGACCCG
References
Reviews
Original Publications
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Time of last update: 2024-09-06 05:49:15
Author of last update: Jstuelk