nupN
168
lipoprotein, part of [metabolite|guanosine] ABC transporter, binding protein
locus
BSU_31540
Molecular weight
37.19 kDa
pI
4.82
function
uptake of [metabolite|guanosine]
product
lipoprotein, part of [metabolite|guanosine] transporter
essential
no
synonyms
nupN, yufN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1744 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,239,930 3,241,009
Phenotypes of a mutant
a ''[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
The protein
Protein family
BMP lipoprotein family (with [protein|4DAB7847EA3848A34432C80124464543FA3DCA0F|med], according to UniProt)
Structure
[PDB|2FQW] (from Treponema pallidum, 45% identity) [pubmed|16418175]
[AF|O05252]
[wiki|Localization]
cell membrane (according to UniProt), membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]
description
[Pubmed|21926227]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21926227], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B553 (yufN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1552 NBRP B. subtilis, Japan]
BKE31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA, downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
BKK31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA, downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
References
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Time of last update: 2024-12-21 18:52:59
Author of last update: Jstuelk