nupN

nupN
168

lipoprotein, part of guanosine transporter

locus
BSU_31540
Molecular weight
37.19 kDa
pI
4.82
Protein length
Gene length
function
uptake of guanosine
product
lipoprotein, part of guanosine transporter
essential
no
synonyms
nupN, yufN

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1744 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,239,930  3,241,009
Phenotypes of a mutant
a ''[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN] [gene|C333F405F597FF260FFA0EC616B8CF6BF454815B|nupG]'' double mutant is unable to utilize externally provided guanosine [Pubmed|21926227]
The protein
Protein family
BMP lipoprotein family (with [protein|4DAB7847EA3848A34432C80124464543FA3DCA0F|med], according to UniProt)
Structure
[PDB|2FQW] (from Treponema pallidum, 45% identity) [pubmed|16418175]
[AF|O05252]
[wiki|Localization]
cell membrane (according to UniProt),  membrane associated [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]-[gene|71546D1508EF17A208332E162CAE2C299987A569|nupO]-[gene|0BB235F189D30C9BE798560C31DA59FF0112CFD9|nupP]-[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]
description
[Pubmed|21926227]
regulation
repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455,21699902,21926227]
regulatory mechanism
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455,21699902,21926227], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21926227], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]→[gene|7A80910F4E62BA15DE93FFC70E1908FB5CB9542D|nupQ]

2024-10-01 17:37:52

ghost

64

176c32eb4a5e0c99af55c2dcae84f2d09f6ef942

1A88DAA8C1BBE629E7600C276F82763A709A3EA7

Biological materials
Mutant
MGNA-B553 (yufN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1552 NBRP B. subtilis, Japan]
BKE31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
BKK31540 ([gene|19BA45BD691BBA4B23C4741C63DB729AA5D30FDA|nupN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31540 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
References
12618455,18763711,20935095,21699902,21926227,16418175

19BA45BD691BBA4B23C4741C63DB729AA5D30FDA

Page visits: 4831

Time of last update: 2024-10-06 01:17:52

Author of last update: Melvin.boenninger