queC
168
synthesis of the modified ribonucleotide queuosine
locus
BSU_13720
Molecular weight
24.38 kDa
pI
5.15
function
tRNA modification
product
unknown
essential
no
synonyms
queC, ykvJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0603 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,439,448 1,440,107
The protein
Catalyzed reaction/ biological activity
7-carboxy-7-deazaguanine + ATP + NH4+ --> 7-cyano-7-deazaguanine + ADP + H+ + H2O + phosphate (according to UniProt)
Protein family
queC family (single member, according to UniProt)
Structure
[PDB|3BL5] [Pubmed|18491386]
[AF|O31675]
Expression and Regulation
Operons
genes
[gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]-[gene|BA6DD5C9AB9BFF9759E9ECC46CE3DF4EB7E67F52|queD]-[gene|F5189CAA5EF43FCF95E593E095F84B3BE486B347|queE]-[gene|5F96500CC46CC4DAECEBAD34EC50B10548E6D0F9|queF]
description
[Pubmed|14660578]
regulation
repressed in the presence of queuosine ([wiki|preQ1 riboswitch]) [Pubmed|19285444]
regulatory mechanism
preQ1 riboswitch: RNA switch, in the absence of queuosine [Pubmed|19285444], in [regulon|other_regulator:preQ1 riboswitch|preQ1 riboswitch]
Biological materials
Mutant
MGNA-A789 (ykvJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/789 NBRP B. subtilis, Japan]
BKE13720 ([gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG
BKK13720 ([gene|1925DFD1162E744E9175D07EC0FD72A75C79C6BF|queC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG
References
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Time of last update: 2025-04-30 21:06:35
Author of last update: Jstuelk