lplD
168
alpha-galacturonidase
locus
BSU_07130
Molecular weight
49.32 kDa
pI
5.36
function
utilization of pectin
product
alpha-galacturonidase
essential
no
synonyms
lplD
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1486 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
782,958 784,298
The protein
Catalyzed reaction/ biological activity
cleavage of alpha-1,4-di-galacturonate [Pubmed|23416295]
[(1→4)-α-D-galacturonosyl](n) + H2O --> [(1→4)-α-D-galacturonosyl](n-1) + α-D-galacturonate (according to UniProt)
Protein family
[wiki|Glycosyl hydrolase 4 family] (according to UniProt)
[wiki|Domains]
active site motif: CHEV [Pubmed|23416295]
[wiki|Cofactors]
NAD, Mn(2+) [Pubmed|23416295]
Structure
[PDB|3FEF]
[AF|P39130]
Expression and Regulation
Operons
genes
[gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]-[gene|EBD0F966FD7B3662814D530064BD307561A5CB15|yetF]
description
[pubmed|22383849]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]: sigma factor, [Pubmed|26577401], in [regulon|protein:CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF regulon]
Biological materials
Mutant
BKE07130 ([gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT
BKK07130 ([gene|18B09A645AC8E13A1AD3B73A0C828E5A0F5D42BB|lplD]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK07130 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTGATATGAAACACTCCCT, downstream forward: _UP4_TGAGCTTGCCGATTAGCTCT
References
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Time of last update: 2024-12-22 07:39:49
Author of last update: Melvin.boenninger