bshA
168
L-malic acid glycosyltransferase, involved in bacillithiol synthesis
locus
BSU_22460
Molecular weight
41.82 kDa
pI
6.15
function
biosynthesis of bacillithiol
product
L-malic acid glycosyltransferase
essential
no
synonyms
bshA, jojH, ypjH
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,357,078 2,358,211
Phenotypes of a mutant
impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
sensitivity to superoxide (paraquate) stress [Pubmed|25988368]
The protein
Catalyzed reaction/ biological activity
UDP-GlcNAc + L-malate --> (S)-malyl N-acetyl-α-D-glucosaminide + H+ + UDP [Pubmed|20308541]; also uses D-malate as a substrate, but with much lower affinity [Pubmed|20308541]
Protein family
[wiki|Glycosyltransferase 1 family] (according to UniProt)
[wiki|Glycosyltransferase 4 subfamily] (according to UniProt)
Structure
[PDB|5D00] [Pubmed|27454321]
[AF|P42982]
Effectors of protein activity
subject to feedback inhibition by bacillithiol [Pubmed|22569254]
Expression and Regulation
Operons
genes
[gene|A828298EA2A002538876FBA6113648AAD9C4C317|ypjD]-[gene|5AB90032031653A913E15B26B3E1AE246E95AABF|dapB]-[gene|341F198B2BD4261B20C00181D084A7C5E33E39B3|mgsA]-[gene|60367F0396612C8A6491FD92A73B30BED7215506|bshB1]-[gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]-[gene|4C4FA59FF5D100E941EF470C0C54E85681ADC74F|cca]-[gene|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]
description
[Pubmed|20308541]
regulation
induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-A410 (ypjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/410 NBRP B. subtilis, Japan]
''bshA::mls'' available in [wiki|John_Helmann] lab
GP88 (''bshA''::''pX2''(''cat'')), available in [wiki|Jörg Stülke]'s lab
BKE22460 ([gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG, downstream forward: _UP4_GATTTAGCAGAACCGGAGTG
BKK22460 ([gene|187C78441816FC74B0FCCE34997E8AEC0A80B114|bshA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG, downstream forward: _UP4_GATTTAGCAGAACCGGAGTG
References
Reviews
Original Publications
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Time of last update: 2024-12-31 08:10:56
Author of last update: Jstuelk