ypeP
168
similar to RNase HI
locus
BSU_21970
Molecular weight
13.58 kDa
pI
5.24
function
unknown
product
unknown
essential
no
synonyms
ypeP
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0328 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,308,967 2,309,647
The protein
[wiki|Domains]
RNase H domain (aa 71-207) (according to UniProt)
Structure
[AF|P54164]
Expression and Regulation
Operons
genes
[gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]-[gene|A4B5C9A4BA04B0501766DADF19A49B67F92857C3|ypeQ]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation] [Pubmed|22383849]
Biological materials
Mutant
MGNA-A893 (ypeP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/893 NBRP B. subtilis, Japan]
BP443 (''ypeP::tet'') available in [wiki|Fabian Commichau]'s lab
BKE21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT, downstream forward: _UP4_TTAGACCGTAACGGAGATGA
BKK21970 ([gene|1852AA40C48859202392834A3E791F6877DD3D30|ypeP]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACAATCACCTTACCAT, downstream forward: _UP4_TTAGACCGTAACGGAGATGA
Expression vectors
for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [wiki|SPINE], based on [wiki|pGP380], expression from plasmid: pBP501 (E. coli amp & B. subtilis E/L) , available in [wiki|Fabian Commichau]'s lab
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Fabian Commichau]'s lab
References
Research papers
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Time of last update: 2024-12-19 14:49:38
Author of last update: Melvin.boenninger