mleA
168
malic enzyme
locus
BSU_23550
Molecular weight
45.73 kDa
pI
4.89
function
[metabolite|malate] utilization
product
[metabolite|NAD+]-dependent [metabolite|malate] dehydrogenase
essential
no
ec
1.1.1.38
synonyms
mleA, yqkJ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0281 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,451,463 2,452,782
The protein
Catalyzed reaction/ biological activity
(S)-[metabolite|malate] + NAD+ --> CO2 + [metabolite|NADH] + [metabolite|pyruvate] (according to UniProt)
H+ + [metabolite|oxaloacetate] --> CO2 + [metabolite|pyruvate] (according to UniProt)
Protein family
[wiki|malic enzymes family] (according to UniProt)
[wiki|Domains]
[wiki|ACT domain] (aa 9-84) (according to UniProt)
Structure
[PDB|2A9F] (from Streptococcus pneumoniae, 42% identity from aa 90 - 435)
[AF|P54572]
Paralogous protein(s)
[protein|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ], [protein|BBBCD5F56779895189E21076AF165B901F654534|malS]
Expression and Regulation
Operons
genes
[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
''[protein|search|ansA]'': expressed in the presence of asparagine ([protein|search|AnsR]) [Pubmed|11914346]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: repression, [Pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|22900538], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1711029], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]-[gene|49DF23B8E19658DB32835B6D23DD21E4BF481B20|mleN]-[gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]
description
[Pubmed|22383849]
regulation
[protein|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]: expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression
expressed in the presence of asparagine ([protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]) [Pubmed|11914346]
expression is reduced in motile cells as compared to non-motile cells [pubmed|33782055]
regulatory mechanism
[protein|146947C974D62BCB282BA3F5B924B35695D75886|ansR]: transcription repression, [pubmed|11914346], in [regulon|protein:146947C974D62BCB282BA3F5B924B35695D75886|ansR regulon]
Biological materials
Mutant
MGNA-C412 (yqkJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2410 NBRP B. subtilis, Japan]
GP1136 (cat) available in [wiki|Jörg Stülke]'s lab
BKE23550 ([gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
BKK23550 ([gene|160EBB7885D7C7FD30A6E35605A862C156E2DA80|mleA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23550 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TATCATTCAGTGATCCTCCC, downstream forward: _UP4_TAAATGTAGCTAAACAGCAC
References
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Time of last update: 2024-11-08 23:05:44
Author of last update: Jstuelk