asnO
168
asparagine synthetase (glutamine-hydrolysing)
locus
BSU_10790
Molecular weight
70.54 kDa
pI
5.7
function
biosynthesis of asparagine
product
asparagine synthetase (glutamine-hydrolysing)
essential
no
ec
6.3.5.4
synonyms
asnO, yisO, yucB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0367 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,157,237 1,159,081
Phenotypes of a mutant
inactivation of ''[gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]'' renders cells asporogenous [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
ATP + H2O + L-aspartate + L-glutamine --> AMP + diphosphate + H+ + L-asparagine + L-glutamate (according to UniProt)
Protein family
asparagine synthetase family (with [protein|6F5BBCBA02EA1C016AA37EB63D252A4C07CE6617|asnB] and [protein|9446D9C88CBBBC5A08495D8AA49A92A9F47F2C9B|asnH], according to UniProt)
[wiki|Domains]
[wiki|Glutamine amidotransferase type-2 domain] (aa 2-216) (according to UniProt)
Structure
[PDB|1CT9] (from E. coli, 27% identity) [pubmed|10587437]
[AF|O05272]
Expression and Regulation
Operons
genes
[gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]
description
[Pubmed|10498721]
regulation
expressed during sporulation ([protein|search|SigE]) [Pubmed|10498721]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|10498721], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
GP4162 (''asnO''::''kan''), available in [wiki|Jörg Stülke]'s lab
BKE10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA
BKK10790 ([gene|15D150B53A25325DBC414F0C2B0D44AEF4B115A9|asnO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGACATCAGCTCCAAT, downstream forward: _UP4_TAGGTTCAATACAAATGTGA
References
Reviews
Original Publications
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Time of last update: 2024-10-12 00:08:47
Author of last update: Jstuelk