kinE
168
two-component sensor kinase, phosphorylates [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F], part of the [wiki|phosphorelay]
locus
BSU_13530
Molecular weight
85.32 kDa
pI
5.49
function
initiation of [wiki|sporulation]
product
two-component sensor kinase
essential
no
synonyms
kinE, ykrQ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5809 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,419,213 1,421,429
The protein
Catalyzed reaction/ biological activity
autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|spo0F]
ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)
[wiki|Domains]
two transmembrane segments
4 [wiki|PAS domain]s (aa 29-99, aa 150-220, aa 271-342, aa 391-462) (according to UniProt)
[wiki|Histidine kinase domain] (aa 523-729) (according to UniProt)
Structure
[PDB|3D36] (Geobacillus stearothermophilus [protein|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB] in complex with the inhibitor [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|sda], corresponds to aa 510 ... 727, 43% identity) [pubmed|19101565]
[AF|O31661]
Modification
autophosphorylation on a His residue
Paralogous protein(s)
[protein|4EE48E4931F662E51586DB6D01E91C688329222D|cssS], [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|yvrG], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|ykoH]
Expression and Regulation
Operons
genes
[gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]-[gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]
description
[Pubmed|2506524]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2506524], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B319 (ykrQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1318 NBRP B. subtilis, Japan]
BKE13530 ([gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA
BKK13530 ([gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA
References
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Time of last update: 2024-12-31 00:13:26
Author of last update: Jstuelk