spoIIM
168
required for dissolution of the septal cell wall
locus
BSU_23530
Molecular weight
24.15 kDa
pI
9.84
function
dissolution of the septal cell wall
product
sporulation protein
essential
no
synonyms
spoIIM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1300 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,450,403 2,451,047
The protein
Structure
[AF|P37873]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
the complex of [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID], [protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM], and [protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] localizes at the leading edge of the mother cell engulfing membrane and is essential and rate limiting for membrane migration [Pubmed|20382772,12502745]
localization of the [protein|AAC4BF6FA80115AB90D2B161C1D5383625953616|spoIID]-[protein|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]-[protein|C9817A54C8E72193280E393D7BD3375BD1751738|spoIIP] complex to the septal membrane depends on [protein|D98D61CA651AAA415386B1B2B41562E5CF77DEF1|spoIIB] [pubmed|36066101]
Expression and Regulation
Operons
genes
[gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]
description
[Pubmed|8501065]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|8501065]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|8501065], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-C416 (spoIIM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2414 NBRP B. subtilis, Japan]
BKE23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC, downstream forward: _UP4_TAAAATCATTATTAAAAGAA
BKK23530 ([gene|14852D54F8B7344AA80D535F8EBBCE11E2B859CF|spoIIM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACCGTTTCCTCCCGCCC, downstream forward: _UP4_TAAAATCATTATTAAAAGAA
References
Reviews
Original Publications
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Time of last update: 2024-09-30 18:33:37
Author of last update: Jstuelk