iolF
168
D-chiro-[metabolite|inositol] transport protein
locus
BSU_39710
Molecular weight
47.03 kDa
pI
9.47
function
D-chiro-inositol uptake
product
D-chiro-inositol transport protein
essential
no
synonyms
iolF, yxdF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG2814 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
4,076,842 → 4,078,158
The protein
Catalyzed reaction/ biological activity
uptake of D-chiro-inositol [Pubmed|20530884]
Protein family
[wiki|major facilitator superfamily] (according to UniProt)
[wiki|Sugar transporter (TC 2.A.1.1) family] (according to UniProt)
Structure
[PDB|4LDS] (from S. epidermidis, 26% identity) [pubmed|24127585]
[AF|P42417]
[wiki|Localization]
cell membrane (according to UniProt)
Expression and Regulation
Operons
genes
[gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
description
[Pubmed|9226270]
regulation
induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]
regulatory mechanism
[protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR]: repression, [Pubmed|9887260,9226270], in [regulon|protein:5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|iolR regulon]
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|10666464], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9226270], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B697 (iolF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1696 NBRP B. subtilis, Japan]
BKE39710 (Δ[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE39710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTTCATTCCCCATTA, downstream forward: _UP4_TAACCAACAGAAGGAGTGGC
BKK39710 (Δ[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK39710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTTCATTCCCCATTA, downstream forward: _UP4_TAACCAACAGAAGGAGTGGC
labs
[wiki|Yasutaro Fujita], University of Fukuyama, Japan
[[Ken-ichi Yoshida]], Kobe University, Japan
References
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Time of last update: 2024-12-21 07:21:28
Author of last update: Jstuelk