spoIIIM
168
sporulation protein, required for the stabilization of septal pore during engulfment
locus
BSU_25060
Molecular weight
10.63 kDa
pI
8.09
function
closure of septal pore
product
single [wiki|LysM domain]-containing protein
essential
no
ec
null
synonyms
yqfZ
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
2,588,701 2,589,000
Phenotypes of a mutant
a [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG] [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM] double mutant has a reduced sporulation efficiency (8.8%) [pubmed|33383000]
a [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG] [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM] double mutant exhibits a severe miscompartmentalization defect [pubmed|33383000]
a [gene|BDC2D775CCE7C06F5619BAC214F29CFC637FE804|pbpG] [gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM] double mutant exhibits severe spore morphological defects [pubmed|33383000]
The protein
Catalyzed reaction/ biological activity
stabilization of septal pore during engulfment [pubmed|33383000]
[wiki|Domains]
N-terminal transmembrane segment [pubmed|33383000]
contains a N-acetylglucosamine-polymer-binding [wiki|LysM domain] (aa 48-95) [Pubmed|18430080]
Structure
[AF|P54483]
[wiki|Localization]
forms a septal focus in the engulfing membrane, this depends on SpoIIIE
Expression and Regulation
Operons
genes
[gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM]-[gene|4992F5C2ED7DE37C08D5715F7E8851F5FDB51059|ispG]
description
[Pubmed|12662922]
regulation
expressed early during sporulation in the mother cell ([protein|search|SigE], [wiki|SpoIIID]) [Pubmed|12662922,15383836]
regulatory mechanism
[protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|15383836], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|12662922], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
MGNA-C488 (yqfZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2486 NBRP B. subtilis, Japan]
BKE25060 ([gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCTTTTCCTCCCGCAT, downstream forward: _UP4_TAATTTAACGTTAATTTCTT
BKK25060 ([gene|144A8B3F5E534F3FD6EFC1E9A5ED650E09DCE885|spoIIIM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGCTTTTCCTCCCGCAT, downstream forward: _UP4_TAATTTAACGTTAATTTCTT
References
Reviews
Original Publications
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Time of last update: 2024-11-29 04:51:47
Author of last update: Jstuelk