yokI
168
LXG toxin, non-specific metal-dependent DNase
locus
BSU_21580
Molecular weight
64.12 kDa
pI
5.76
function
competetion with other bacteria in biofilms
product
LXG toxin, DNase
essential
no
ec
null
synonyms
yokI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG5444 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,275,706 2,277,421
Phenotypes of a mutant
the mutant is outcompeted by wild type cells in biofilms [pubmed|34280190]
The protein
[wiki|Domains]
[wiki|LXG domain] (aa 1-235) (according to UniProt)
Structure
[AF|O31998]
[wiki|Localization]
secreted
secretion and delivery requires [protein|50D2A03E2D7B5D461540FD157377E0D37F771888|WXG100] and the T7SS ([protein|3CC3E197848E9688413C15CEA7DDCF0A7120A920|yukD]-[protein|3FE4A91C7D0B43C8704391F2F9493852B3AED9B0|essB]-[wiki|YukB]-[wiki|YueB]-[wiki|YueC]-[protein|B258CA26B0EC2310475FCCD67F1F196857FC0004|yueD]) [pubmed|34280190]
Expression and Regulation
Operons
genes
[gene|13C081250872E72C61C49D8F77AC5AF4B6D431A3|yokI]-[gene|9BC2309D7FC93CD4195849BFCF195ED3EBC3CAF1|yokJ]-[gene|8BA8DB1483190AB847F28F3E56C247FE85C3225E|yokK]-[gene|9A03964C94A9253B3D9AAA60ACA0651AED49A627|yokL]
description
[Pubmed|20817675]
regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yolA]' and '[protein|search|yokL]' [PubMed|20525796]
Biological materials
Mutant
BKE21580 ([gene|13C081250872E72C61C49D8F77AC5AF4B6D431A3|yokI]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTCTCCTTTCAAG, downstream forward: _UP4_AACTTTAGAAAGTAGGTGCG
BKK21580 ([gene|13C081250872E72C61C49D8F77AC5AF4B6D431A3|yokI]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCATTCTCCTTTCAAG, downstream forward: _UP4_AACTTTAGAAAGTAGGTGCG
References
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Time of last update: 2024-12-30 16:14:16
Author of last update: Jstuelk