yloM
168
similar to RNA-binding Sun protein, similar to S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase
locus
BSU_15740
Molecular weight
50.11 kDa
pI
6.95
function
rRNA modification
product
S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase
essential
no
synonyms
yloM, sun
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0781 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,647,939 1,649,282
The protein
Catalyzed reaction/ biological activity
cytidine967 in 16S rRNA + S-adenosyl-L-methionine --> 5-methylcytidine967 in 16S rRNA + H+ + S-adenosyl-L-homocysteine (according to UniProt)
Protein family
[wiki|Methyltransferase superfamily] (according to UniProt)
[wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)
Structure
[PDB|1SQF] (''E. coli'' Fmu protein, complex with SAM) [Pubmed|14656444]
[AF|P94464]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[Pubmed|16964327]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16964327], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|coaBC]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B373 (yloM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1372 NBRP B. subtilis, Japan]
BKE15740 ([gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC, downstream forward: _UP4_AGCATGAGAAAGAAGGGATA
BKK15740 ([gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC, downstream forward: _UP4_AGCATGAGAAAGAAGGGATA
References
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Time of last update: 2024-12-21 14:55:00
Author of last update: Jstuelk