nadC
168
nicotinate-nucleotide diphosphorylase (carboxylating)
locus
BSU_27860
Molecular weight
31.24 kDa
pI
5.18
function
NAD biosynthesis
product
nicotinate-nucleotide diphosphorylase (carboxylating)
essential
no
ec
2.4.2.19
synonyms
nadC, yrxB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0157 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,847,048 2,847,917
The protein
Catalyzed reaction/ biological activity
CO2 + diphosphate + nicotinate β-D-ribonucleotide --> 5-phospho-α-D-ribose 1-diphosphate + 2 H+ + quinolinate (according to UniProt)
Protein family
nadC/modD family (single member, according to UniProt)
Structure
[PDB|1X1O] (from Thermus thermophilus, 45% identity)
[AF|P39666]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB]-[gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]-[gene|F92F17578F06EF2292D0923662933463D7C86498|nadA]
description
[Pubmed|8444804]
regulation
repressed in the presence of nicotinic acid ([protein|search|NadR]) [Pubmed|16199587]
regulatory mechanism
[protein|510DE075EA964D22C42DEB171509F6CDA600A402|nadR]: repression, [Pubmed|16199587], in [regulon|protein:510DE075EA964D22C42DEB171509F6CDA600A402|nadR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8444804], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
An [wiki|search|ncRNA] is predicted for '[protein|search|nadB]' [PubMed|20525796]
Biological materials
Mutant
BKE27860 ([gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCAAGAAAAAAGTGATTC, downstream forward: _UP4_TGTTATGTCAATTCTTGATG
BKK27860 ([gene|1368734DCC28C618AA173B865784BDDAEA912623|nadC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCTCAAGAAAAAAGTGATTC, downstream forward: _UP4_TGTTATGTCAATTCTTGATG
References
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Time of last update: 2025-04-30 19:29:11
Author of last update: Melvin.boenninger