dhbB
168
isochorismatase
locus
BSU_31970
Molecular weight
34.95 kDa
pI
4.43
function
biosynthesis of the siderophore bacillibactin
product
isochorismatase
essential
no
ec
3.3.2.1
synonyms
dhbB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG3433 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,287,675 3,288,613
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium [pubmed|31420537]
The protein
Catalyzed reaction/ biological activity
Isochorismate + H2O --> 2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate (according to UniProt)
Protein family
[wiki|Isochorismatase family] (according to UniProt)
[wiki|Domains]
[wiki|Carrier domain] (aa 229-302) (according to UniProt)
Structure
[PDB|2FQ1] (from E. coli, 49% identity) [pubmed|16632253]
[AF|P45743]
Expression and Regulation
Operons
genes
[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
description
[Pubmed|8921902]
regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
expression is increased in the presence of pulchimerrin [pubmed|37137890]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8550523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
Biological materials
Mutant
BKE31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
BKK31970 ([gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGAACACTTCCTCTCA, downstream forward: _UP4_TTATAAAAGGAGGGTAAACA
References
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Time of last update: 2024-12-26 00:45:39
Author of last update: Jstuelk