prfB

prfB
168

peptide chain release factor 2, recognize stop codon and hydrolyze the completed protein, RF2 recognizes UAA and UGA

locus
BSU_35290
Molecular weight
41.91 kDa
pI
4.85
Protein length
Gene length
function
translation
product
peptide chain release factor 2
essential
yes
synonyms
prfB

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1186 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,627,139  3,628,240
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA [pubmed|11158343]
Protein family
prokaryotic/mitochondrial release factor family (with [protein|59E6468FD891F5271F38543515867B0B4625768D|prfA], according to UniProt)
Structure
[PDB|1MI6] (from ''E. coli'', 45% identity) [Pubmed|12511960]
[AF|P28367]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
description
[Pubmed|9882663]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]→[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]

2024-09-21 03:39:59

Bzhu

90

00efa3c81b1b91aca22f058bbac28569ff967366

43AB19862CD02CECE51182E4F38F545D7AEAC1D6

References
Reviews
11158343
Original Publications
9882663,20512119,25217634,12511960,33900014

12E06FD3A36D95AE82E3C65788FA98178039592F

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Time of last update: 2024-10-03 00:05:28

Author of last update: Jstuelk