prfB
168
peptide chain release factor 2, recognize stop codon and hydrolyze the completed protein, RF2 recognizes UAA and UGA
locus
BSU_35290
Molecular weight
41.91 kDa
pI
4.85
function
translation
product
peptide chain release factor 2
essential
yes
synonyms
prfB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1186 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,627,139 3,628,240
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
binds to the ribosome through specific recognition of messenger RNA stop codons and triggers hydrolysis of the bond between the nascent polypeptide and the transfer RNA at the peptidyl-tRNA site, thereby releasing the newly synthesized protein. PrfB is highly specific for a U in the first stop-codon position and recognizes UAA and UGA [pubmed|11158343]
Protein family
prokaryotic/mitochondrial release factor family (with [protein|59E6468FD891F5271F38543515867B0B4625768D|prfA], according to UniProt)
Structure
[PDB|1MI6] (from ''E. coli'', 45% identity) [Pubmed|12511960]
[AF|P28367]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|AD7CD451B4AE638A719C91780339784A7FF0F248|secA]-[gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB]
description
[Pubmed|9882663]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|9882663], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
the [gene|12E06FD3A36D95AE82E3C65788FA98178039592F|prfB] gene contains an in-frame premature RF2-specific stop codon that is important to control the levels of the protein [pubmed|39386688]
References
Reviews
Original Publications
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Time of last update: 2024-12-15 07:18:58
Author of last update: Jstuelk