spoIIIE
168
ATP-dependent dsDNA translocase, resolution of chromosomal dimers after [wiki|DNA replication], coordinates chromosome segregation and peptidoglycan remodeling during sporulation, transports the forespore chromosome across the [wiki|sporulation] septum
locus
BSU_16800
Molecular weight
86.96 kDa
pI
6.76
function
chromosome partition during [wiki|sporulation]
product
ATP-dependent dsDNA translocase required for chromosome partition
essential
no
synonyms
spoIIIE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1674 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,752,272 1,754,641
The protein
Catalyzed reaction/ biological activity
transports the forespore chromosome across the [wiki|sporulation ]septum [Pubmed|20516200]
translocates septum-entrapped DNA only when septum closure precedes complete segregation of chromosomes [Pubmed|19818024]
the two DNA translocases [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA] and [protein|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE] synergistically affect chromosome dimer resolution presumably by positioning the dif sites in close proximity, before or after completion of cell division, respectively [Pubmed|21239579]
binds DNA sequences named SRS for SpoIIIE recognition sequence, GAGAAGGG [Pubmed|18391964]
Protein family
FtsK/SpoIIIE/SftA family (with [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA], according to UniProt)
[wiki|Domains]
N-terminal transmembrane domain (4 helices) [Pubmed|24297254]
134 aa linker [Pubmed|24297254]
512 aa motor domain at the C-terminus [Pubmed|24297254]
[wiki|FtsK domain] (aa 450-646) (according to UniProt)
Structure
[PDB|2IUT] (FtsK from ''Pseudomonas aeruginosa'', partial, 48% identity, complex with [protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]) [Pubmed|16916635]
[AF|P21458]
Paralogous protein(s)
[protein|DF77B46666595E86081C5345C91E08F86DF9FAEE|sftA]
[wiki|Localization]
free diffusion in the membrane, no enrichment at septa [pubmed|29439991]
transmembrane domain mediates dynamic localization to active division sites [Pubmed|20516200]
complexes are recruited to nascent sites of septation, and are subsequently escorted by the constriction machinery to the center of [wiki|sporulation] and division septa [Pubmed|23667326]
anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum [pubmed|33383000]
Expression and Regulation
Operons
genes
[gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]-[gene|F9E0C645BCCD9A299E951D0574321C1FACA8DE4C|ymfC]
description
[Pubmed|22383849]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2507870], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
BKE16800 ([gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
BKK16800 ([gene|125325B485FBC60798EDA4B4BFB24C6FCBC4C513|spoIIIE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACACTCATCACCTTACTGC, downstream forward: _UP4_TAATGAAGGGAGTTCCGCTT
References
Reviews
Original Publications
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Time of last update: 2024-12-30 03:29:23
Author of last update: Jstuelk