floA
168
flottilin-like protein (in addition to [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]), reduces membrane order, resistence protein (against sublancin), accessory role in resistance to cefuroxime
locus
BSU_25380
Molecular weight
35.48 kDa
pI
4.92
function
control of membrane fluidity
product
flottilin-like protein
essential
no
synonyms
floA, yqfA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4864 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,617,449 2,618,444
Phenotypes of a mutant
reduced [wiki|protein secretion] [Pubmed|23651456]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant does not induce [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|kinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
a ''[gene|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] [gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' double mutant has a [wiki|sporulation] defect, due to the lack of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] [Pubmed|22882210]
a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant produces increased amount of pulcherriminic acid [Pubmed|25909364]
a ''[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]'' mutant has reduced sensitivity to vancomycin [Pubmed|25909364]
The protein
Catalyzed reaction/ biological activity
controls protease activity of [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|ftsH] [Pubmed|24222488]
Protein family
Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with [protein|CA4EF8385EAA4BBC47FF0D998C820D9F36F5F94D|ydjI] and [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT]) [pubmed|34777311]
UPF0365 family (single member, according to UniProt)
[wiki|Domains]
contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 42 - 222) [pubmed|34777311]
Structure
[AF|P54466]
[wiki|Localization]
membrane [Pubmed|18763711]
co-localizes with [protein|61893B4EB53FBBB9AD8BEEEFFCEFD0FC64544D6F|floT] in discrete foci in the membrane [Pubmed|20713508]
forms focal structure in the cytoplasma membrane [Pubmed|22753055]
13 foci [protein|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA] of are detected in strain 3610 [Pubmed|25909364]
Expression and Regulation
Operons
genes
[gene|F6B0D27B434D81B7A680555E4457F266B6206B7D|yqeZ]-[gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]-[gene|A80317C3B8DF06E2187E02A05C13845508C18B40|yqfB]
description
[Pubmed|11866510]
regulation
expressed under stress conditions ([protein|search|SigW]) [Pubmed|11866510]
sigma factors
[protein|3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW]: sigma factor, [Pubmed|11866510], in [regulon|protein:3D974DD2967C7F8FD4E3C2AF42617B8AE9F0296D|sigW regulon]
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
MGNA-C443 (yqfA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2441 NBRP B. subtilis, Japan]
JS119 (3610 markerless), available in [wiki|Daniel Lopez]'s lab
DL1401 (168 ''floA''::mls), available in [wiki|Daniel Lopez]'s lab
BKE25380 ([gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG
BKK25380 ([gene|11A55829B4C3E8EA434A600FF7B4102F8E5A7DCE|floA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK25380 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATAACTTCTCCTCGTTT, downstream forward: _UP4_TAATCTCTCTAAGAAGGGAG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]) for FloA [Pubmed|25909364], available in [wiki|Daniel Lopez]'s lab
GFP fusion
GK192 (3610 ''lacA''::P''floT-yfp''(mls)), available in [wiki|Daniel Lopez]'s lab
JS136 (3610 ''amyE::floT''-gfp(spc)), available in [wiki|Daniel Lopez]'s lab
JS134 (3610 ''lacA::floA''-mEos2 (mls)), available in [wiki|Daniel Lopez]'s lab
JS167 (3610 ''lacA::floA''-PAmCherry (mls)), available in [wiki|Daniel Lopez]'s lab
References
Reviews
Original Publications
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Time of last update: 2025-04-02 08:26:12
Author of last update: Jstuelk