bshC
168
cysteine-adding enzyme required for the synthesis of bacillithiol
locus
BSU_15120
Molecular weight
62.44 kDa
pI
5.25
function
biosynthesis of bacillithiol
product
cysteine-adding enzyme
essential
no
synonyms
bshC, yllA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG4365 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,578,376 1,579,995
Phenotypes of a mutant
impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster [Pubmed|25988368]
sensitivity to superoxide (paraquate) stress [Pubmed|25988368]
The protein
Protein family
BshC family (single member, according to UniProt)
Structure
[PDB|4WBD] [Pubmed|25496067]
[AF|P55342]
Expression and Regulation
Operons
genes
[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
description
[Pubmed|20308541]
regulation
''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Biological materials
Mutant
MGNA-B128 (bshC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1127 NBRP B. subtilis, Japan]
a ''bshC'' null mutant is available in [wiki|John Helmann]s lab
BKE15120 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
BKK15120 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTCTAGAACTTCCTTT, downstream forward: _UP4_TGAAATAAAGTTTTAAAGAA
BP953 ([gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]::cat ([gene|651D1E534490276B9BE7BFE7B5819176AE09891E|pdxS]-[gene|6F96C10759C1C20B3A6B396E3718F0A9281F100A|pdxT])::tet), available in [wiki|Fabian Commichau]'s lab, [Pubmed|29027347]
References
Reviews
Original Publications
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Time of last update: 2024-12-05 18:38:12
Author of last update: Melvin.boenninger