cwlS
168
minor autolysis, D,L-endopeptidase, peptidoglycan hydrolase for cell separation
locus
BSU_19410
Molecular weight
44.07 kDa
pI
10.44
function
cell separation
product
D,L-endopeptidase, peptidoglycan hydrolase
essential
no
synonyms
cwlS, yojL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1388 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,115,425 2,116,669
The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
degradation of gamma-polyglutamic acid [pubmed|29458655]
Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A(2)pm) in 7-(L-Ala-gamma-D-Glu)-meso-A(2)pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A(2)pm. It is required that the D-terminal amino and carboxy groups of meso-A(2)pm are unsubstituted (according to UniProt)
Protein family
[wiki|Peptidase C40 family] (according to UniProt)
[wiki|Domains]
contains four N-acetylglucosamine-polymer-binding [wiki|LysM domain]s [Pubmed|24355088,18430080]
C-terminal D,L-endopeptidase domain ([wiki|NlpC/P60 domain]) [pubmed|29458655,22139507]
4 [wiki|LysM domain]s (aa 27-70, aa 88-131, aa 157-200, aa 225-268) (according to UniProt)
Structure
[PDB|4XCM] (from Thermus thermophilus, 34% identity) [pubmed|25760608]
[AF|O31852]
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [protein|0CA55371306D4BD768CFA82027DCB4D581BCCB87|iseA] [pubmed|29458655]
[wiki|Localization]
localizes to cell septa and poles [Pubmed|22139507]
Expression and Regulation
Operons
genes
[gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]
description
[pubmed|22383849]
regulation
repressed by glucose (3-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [pubmed|], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh]: repression, synergistic transcription repression with [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB] [Pubmed|20817675], in [regulon|protein:5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, synergistic transcription repression with [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|abh] [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|sigD]: sigma factor, [pubmed|22139507], in [regulon|protein:7024E4162A6D827069F882FDEACA696EBC05DD40|sigD regulon]
[protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH]: sigma factor, [pubmed|22139507], in [regulon|protein:DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|sigH regulon]
genes
[gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]-[gene|3B45734D18D01BAB6D244DE3547C84F9488075FB|yojM]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B424 (yojL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1423 NBRP B. subtilis, Japan]
BKE19410 ([gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
BKK19410 ([gene|0FBE3F7ECACA7FCFAA4EE32E9B2BB35F0B793C0F|cwlS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19410 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCATTCAACCTCCTAAA, downstream forward: _UP4_TAAAAATGAGTATGACAAAA
References
Reviews
Original Publications
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Time of last update: 2025-01-26 14:07:02
Author of last update: Jstuelk