panE

panE
168

[metabolite|ketopantoate] reductase

locus
BSU_15110
Molecular weight
33.13 kDa
pI
5.86
Protein length
Gene length
function
biosynthesis of [metabolite|coenzyme A]
product
[metabolite|ketopantoate] reductase
essential
no
ec
1.1.1.169
synonyms
ylbQ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1893 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,577,409  1,578,305
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
[metabolite|ketopantoate] + H+ + [metabolite|NADPH] --> (R)-[metabolite|pantoate] + [metabolite|NADP]+ [pubmed|39194188]
Protein family
ketopantoate reductase family (with [protein|60264E4BE76F5995A8317294D05FC27F8B34E09D|panG], according to UniProt)
Structure
[PDB|3EGO]
[AF|O34661]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
genes
[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]-[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]
description
[Pubmed|20308541]
regulation
''[protein|search|ylbQ]'': induced by diamide stess (thiol depletion) ([protein|search|Spx]) [Pubmed|23894131]
regulatory mechanism
[protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|23894131], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|23894131], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
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[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]→[gene|115FA333FAD17ACE5EBF288F16F33C330C6B4473|bshC]

2024-10-14 07:52:21

ghost

88

5167d69519c79cbaec57ca3a2697ba19d8c2a92d

EAD0EECB1A87533845507CEC4FCC017629071F4A

Biological materials
Mutant
MGNA-B254 (ylbQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1253 NBRP B. subtilis, Japan]
BKE15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT,  downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
BKK15110 ([gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15110 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGATGTCACACCCAATTT,  downstream forward: _UP4_TGAGCTTTTTCGGTAACATG
GP3384 (Δ[gene|0F153803781C9AC8321FB14F926B645C45E53B00|panE]::neo trpC2), available in [wiki|Jörg Stülke]'s lab [pubmed|39194188]
References
Original Publications
20308541,23894131,39194188

0F153803781C9AC8321FB14F926B645C45E53B00

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Time of last update: 2024-10-15 03:21:54

Author of last update: Jstuelk