sdhA
168
succinate dehydrogenase (flavoprotein subunit)
locus
BSU_28440
Molecular weight
65.17 kDa
pI
5.71
function
TCA cycle
product
succinate dehydrogenase (flavoprotein subunit)
essential
no
synonyms
sdhA, citF
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1053 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,906,335 2,908,095
Phenotypes of a mutant
severe growth inhibition upon addition of subinhibitory concentrations of mirubactin C [pubmed|36312962]
The protein
Catalyzed reaction/ biological activity
menaquinone + succinate --> menaquinol + fumarate [pubmed|38287013]
menaquinone + malate --> menaquinol + enol-oxaloacetate (minor side reaction) [pubmed|38287013]
Protein family
FAD-dependent oxidoreductase 2 family (with [protein|D30D059185DFB828AB0FD07D600DBC3FD0973198|nadB], according to UniProt)
[wiki|Cofactors]
Fe
FAD (according to UniProt)
Structure
[PDB|1NEK] (''E. coli'') [pubmed|12560550]
[AF|P08065]
Modification
phosphorylated [Pubmed|16493705], [Pubmed|17726680]
Effectors of protein activity
Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [Pubmed|3910107]
Inhibited by enroll-oxaloacetate [pubmed|38287013]
Activated by Cytochrome b558 [Pubmed|6799760]
[wiki|Localization]
attached to the membrane [Pubmed|18763711]
Expression and Regulation
Operons
genes
[gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB]
description
[Pubmed|3036777]
regulation
constitutive
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2495271], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Other regulations
[protein|CE542AE1A32CCD1ABB259E6159F7A37A8078E189|fsrA]: translation inhibition, [Pubmed|22389480]
Biological materials
Mutant
GP743 ∆([gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]), cat, available in [wiki|Jörg Stülke]'s lab
GP792 ∆([gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB])::''phleo'', available in [wiki|Jörg Stülke]'s lab
GP2342 ∆([gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB])::''kan'', Cre-recombinase is integrated in [gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], available in [wiki|Jörg Stülke]'s lab
GP2343 ∆([gene|81429F7802CD4B1F7DFDB6C900D7DA5ECA6B9F3F|sdhC]-[gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]-[gene|901F90AFC94CC7140709845333416106D043269D|sdhB])::''lox72'', Cre-recombinase is integrated in [gene|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|sacA], available in [wiki|Jörg Stülke]'s lab
BKE28440 ∆([gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA])::erm trpC2, available at [http://bgsc.org/getdetail.php?bgscid=BKE28440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
BKK28440 ∆([gene|0E1FAF4B3A967CC3882B3DDB3CA2D01FD70A0F7E|sdhA]::kan) trpC2, available at [http://bgsc.org/getdetail.php?bgscid=BKK28440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAGCCCCTCTCCCTC, downstream forward: _UP4_AAGAAGAAGGTGGCGAAATA
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-11-21 03:50:45
Author of last update: Jstuelk