sndA
168
sulphur compound N-deacetylase, required for the conversion of S-methyl-cysteine to cysteine
locus
BSU_29290
Molecular weight
45.08 kDa
pI
5.55
function
utilization of S-methyl-cysteine
product
sulphur compound N-deacetylase
essential
no
synonyms
sndA, hipO, ytnL
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1473 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,999,717 3,000,967
Phenotypes of a mutant
a [gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA] [gene|C99AA3FDBB561BCED60915078DC746690C071753|sndB] [gene|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC] triple mutant does not grow with N-acetyl cysteine and S-(2-succino)cysteine as the single sources of sulfur [Pubmed|23944997,29626092]
The protein
Catalyzed reaction/ biological activity
deacetylation of N-acetyl-l-cysteine in a Zn2+- or Co2+-dependent reaction [pubmed|35584544]
Protein family
[wiki|peptidase M20 family] (according to UniProt)
Structure
[PDB|1YSJ] ([protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], 48% identity)
[AF|O34980]
Paralogous protein(s)
[protein|C99AA3FDBB561BCED60915078DC746690C071753|sndB], [protein|D1A8D231369F0BF48639B61094B3222EC4605B79|sndC], [protein|D6F1906CE8386F78F451D6B794F74DE4AD25AC9C|ykuR]
Expression and Regulation
Operons
genes
[gene|E3F1B3A4086DBC40FD9AE9CD6981270FBDFA6CAF|snaA]-[gene|A6A13C54177381CE78ABA2CFC2A3F2CE12099F15|tcyJ]-[gene|A8FA40EACDB15CF53DDDA725A3AA5A6EB654FA8B|tcyK]-[gene|350C97E4A4C60A6E2FF9610D50B4A49FB9F9B42D|tcyL]-[gene|B1DD32A3B3D1C0C5391A40834940257357A2F30F|tcyM]-[gene|B24763A732111026D21C828FF9BE73FB22A123CF|tcyN]-[gene|EDC3565D90D7059A4A4C66A13EB568420F6CECD4|cmoO]-[gene|FCD25CA4414D689B217F54B0C44EB805F9590454|cmoI]-[gene|8145952307BC47805A7A9BD39418474358043BFE|cmoJ]-[gene|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|ribR]-[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]-[gene|910EAAF48C85D0364751EC9585121246AF677431|ytnM]
description
[Pubmed|15668000]
regulation
induced in the presence of methionine and taurine [Pubmed|11390694]
regulatory mechanism
[protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16109943], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]
[protein|741156D495BE3857683C8A0390764EAD83845ABC|ascR]: activation, [Pubmed|16109943], in [regulon|protein:741156D495BE3857683C8A0390764EAD83845ABC|ascR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15272571], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
[protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]
Biological materials
Mutant
GP4474 (trpC2 Δ[gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::neo), available in [wiki|Jörg Stülke]'s lab
BKE29290 ([gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGACATATGTTCCGCTCCTT, downstream forward: _UP4_GATTGATATAGATGGGGTGT
BKK29290 ([gene|0DE66A10D9475DC57A140561E8532D3EC038FD6F|sndA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29290 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGACATATGTTCCGCTCCTT, downstream forward: _UP4_GATTGATATAGATGGGGTGT
References
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Time of last update: 2024-12-19 23:22:20
Author of last update: Robert.warneke