pchR
168
transcriptional repressor ([wiki|MarR family]), controls the expression of genes involved in pulcherriminic acid biosynthesis
locus
BSU_35080
Molecular weight
19.54 kDa
pI
8.09
function
control of pulcherriminic acid biosynthesis
product
transcription factor ([wiki|MarR family])
essential
no
synonyms
pchR, yzhA, yvmB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,604,993 3,605,502
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
inactivation of ''[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]'' causes widespread disruption of proteins involved in cellular processes that depend on iron availability, with pleiotropic effects on proteins associated with carbon metabolism, translation, stress response, biosynthesis of cell wall, and sporulation initiation [Pubmed|27542896]
The protein
Protein family
[wiki|MarR family] of transcriptional regulators [Pubmed|27542896]
[wiki|Domains]
[wiki|HTH marR-type domain] (aa 10-153) (according to UniProt)
Structure
[AF|P40762]
Paralogous protein(s)
[protein|F5A59CE7727E0851652BB4C0144009154AB4978B|yhjH]
Additional information
Proposed consensus sequence for DNA-binding site: GTTYMMYMGTAAAC [Pubmed|27542896]
Expression and Regulation
Operons
genes
[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]-[gene|7F10DEEDEF8B679F4F2C5EAD5752F4E399DB0D57|yvmA]
description
[Pubmed|22383849]
regulation
induced by iron starvation [Pubmed|27542896]
regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135]; [protein|0D555F2AB7DC863E6FF388888308E980514DB719|pchR] auto-repression [Pubmed|27542896], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]
[protein|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]: repression, in [regulon|protein:0D555F2AB7DC863E6FF388888308E980514DB719|pchR regulon]
Biological materials
Mutant
CS217 (''[gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]''::''aphA3'', available in [wiki|Colin Harwood]'s and [wiki|Jörg Stülke]'s lab [Pubmed|27197833]
BKE35080 ([gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE35080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTACCTTCTTTCTT, downstream forward: _UP4_TAAACAAAAAGGCGGTGTAC
BKK35080 ([gene|0D555F2AB7DC863E6FF388888308E980514DB719|pchR]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK35080 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGGCTACCTTCTTTCTT, downstream forward: _UP4_TAAACAAAAAGGCGGTGTAC
labs
Sandrine Auger, Micalis Institute, INRA, AgroParisTech, France
References
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Time of last update: 2025-04-03 14:28:48
Author of last update: Jstuelk