dhbC
168
isochorismate synthase
locus
BSU_31990
Molecular weight
43.29 kDa
pI
5.17
function
biosynthesis of the siderophore bacillibactin
product
isochorismate synthase
essential
no
ec
5.4.4.2
synonyms
dhbC
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1169 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,290,289 3,291,485
Phenotypes of a mutant
defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium [pubmed|31420537]
The protein
Catalyzed reaction/ biological activity
Chorismate --> isochorismate (according to UniProt)
Protein family
isochorismate synthase family (with [protein|8EFE4D2B07E54A0EE7D6821C9FEBFC7DC22FDE03|menF], according to UniProt)
Structure
[PDB|3OS6], (from ''B. anthracis'', 56% identity, 70% similarity)
[AF|P45744]
Modification
phosphorylation on Ser-271 [Pubmed|17218307]
Expression and Regulation
Operons
genes
[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]-[gene|DD647083C2E6968AD26A41C9F4EFBA942D75207C|ybdZ]
description
[Pubmed|8921902]
regulation
induced by iron starvation (second wave to allow iron scavenging from the environment) ([protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]) [Pubmed|29133393,12354229]
expression is increased in the presence of pulchimerrin [pubmed|37137890]
expression
expression is heterogeneous [pubmed|35171018]
regulatory mechanism
[protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur]: repression, [pubmed|12354229], in [regulon|protein:F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]: repression, in [regulon|protein:65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8550523], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
[protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI]: sigma factor, [pubmed|29914988], in [regulon|protein:3DEAC421B4B173C6BBC700E57790751B7AFDF319|sigI regulon]
additional information
the ''[gene|42C8D303AABFA2A9706295A0F90B0B49F854B0BF|dhbA]-[gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]-[gene|B864EC6D21AD5E524AC13AE1BE41F5CD7FC399C5|dhbE]-[gene|1346D57F906EE5BD36F7FBFA1E4884EEBC8585FA|dhbB]-[gene|95B75CFF126AF0AD845E62B4036DD3B6DAC8C7FA|dhbF]'' operon is strongly unregulated in a ''[wiki|kre]'' mutant [Pubmed|26110430]
Biological materials
Mutant
BKE31990 ([gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE31990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCATGAACCTCCTTT, downstream forward: _UP4_TGAACAGAATGAATTGCTGG
BKK31990 ([gene|0D4D200095AD4F9B30179B9AF500306EDA61F5D1|dhbC]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK31990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCATGAACCTCCTTT, downstream forward: _UP4_TGAACAGAATGAATTGCTGG
References
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Time of last update: 2024-12-30 08:21:50
Author of last update: Jstuelk