tmcAL
168
tRNAMet cytidine acetate ligase, required for proper decoding of the AUA elongator Met codon
locus
BSU_15060
Molecular weight
46.83 kDa
pI
7.8
function
acetylation of elongator tRNAMet
product
tRNAMet cytidine acetate ligase
essential
no
synonyms
tmcAL, ylbM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1323 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,573,807 1,575,054
Phenotypes of a mutant
cold-sensitive [pubmed|30150682]
restores viability of a ''[gene|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc]'' mutant on rich medium due to the induction of a [wiki|stringent response] [Pubmed|26539825]
defective in swarming motility (reduced rate of colony expansion) [pubmed|35638827]
The protein
Catalyzed reaction/ biological activity
acetylation of the first position of the anticodon (position 34) of elongator tRNAMet [pubmed|30150682]
acetate + ATP + cytidine34 in elongator tRNAMet --> AMP + diphosphate + N4-acetylcytidine34 in elongator tRNAMet (according to UniProt)
Protein family
TmcAL family (single member, according to UniProt)
Structure
[PDB|5Y0O] (apo-form) [pubmed|30150682]
[PDB|5Y0P] (bound to alpha-thio ATP) [pubmed|30150682]
[PDB|5Y0Q] (bound to AMPCPP) [pubmed|30150682]
[PDB|5Y0N] (selenomethionine derivative) [pubmed|30150682]
[AF|O34513]
Expression and Regulation
Operons
genes
[gene|0311601A6499C5B5A84690242132AC4AC1D36B26|ylzH]-[gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]
description
[pubmed|22383849]
Biological materials
Mutant
MGNA-B250 (ylbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1249 NBRP B. subtilis, Japan]
BKE15060 ([gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT
BKK15060 ([gene|0C76CAE27ACEBC0FF6B864B9F8C7D3C41C8D2A24|tmcAL]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15060 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTCAACCACCAATCCGACAG, downstream forward: _UP4_AATGTATAGAAAAAGCACCT
References
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Time of last update: 2024-12-21 22:38:54
Author of last update: Jstuelk