nahA
168
RNA pyrophosphohydrolase, converts primary transcripts to 5’-monophosphate RNA
locus
BSU_34780
Molecular weight
18.07 kDa
pI
4.99
function
initiation of RNA degradation
product
RNA pyrophosphohydrolase
essential
no
synonyms
nahA, yvcI
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1051 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,572,413 3,572,889
The protein
Catalyzed reaction/ biological activity
converts primary transcripts to 5’-monophospate RNA [pubmed|31740579]
prefers G-initiating RNAs and required at least one unpaired nucleotide at the 5’-end of its substrates, with the 5’-terminal nucleotide determining whether primarily ortho- or pyrophosphate is released [pubmed|31740579]
Protein family
[wiki|Nudix hydrolase] (according to UniProt)
[wiki|Domains]
[wiki|Nudix hydrolase domain] (aa 3-130) (according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|31740579]
Structure
[PDB|3FK9] (the protein from ''B. halodurans'', 59% identity/ 82% similarity)
[AF|O06972]
Expression and Regulation
Operons
genes
[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
description
[Pubmed|9237995]
regulation
constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]
expression
in Enterococcus faecalis: induced upon cell wall stress (vancomycin) ([protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]) [pubmed|38512984]
regulatory mechanism
[protein|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]: transcription repression, described for Enterococcus faecalis [pubmed|38512984], in [regulon|protein:1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|16272399], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]' [PubMed|20525796]
Biological materials
Mutant
MGNA-B642 (yvcI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1641 NBRP B. subtilis, Japan]
BKE34780 (Δ[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
BKK34780 (Δ[gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34780 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCTAACCTTCGTCCTGTC, downstream forward: _UP4_TAGAAAGACAAGTCAGGGGG
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-10-05 11:15:46
Author of last update: Jstuelk