pgpB
168
phosphatidylglycerol phosphate (PGP) phosphatase , has minor undecaprenyl pyrophosphate phosphatase activity
locus
BSU_19650
Molecular weight
22.64 kDa
pI
10.14
function
phospholipid biosynthesis
product
phosphatidylglycerol phosphate (PGP) phosphatase
essential
no
synonyms
yodM
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0671 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,136,913 2,137,524
The protein
Catalyzed reaction/ biological activity
1,2-diacyl-sn-glycero-3-phospho-(1ʼ-sn-glycero-3ʼ-phosphate) + H2O --> 1,2-diacyl-sn-glycero-3-phospho-(1'-sn-glycerol) + phosphate (according to UniProt)
Protein family
PA-phosphatase related phosphoesterase family (single member, according to UniProt)
Structure
[PDB|5JKI] [Pubmed|28168443]
[AF|O34349]
[wiki|Localization]
cell membrane (integral) [Pubmed|28168443]
Expression and Regulation
additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
GP3652 Δ[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::cat, available in [wiki|Jörg Stülke]'s lab
GP3680 Δ[gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::aphA3, available in [wiki|Jörg Stülke]'s lab
MGNA-B441 (pgpB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1440 NBRP B. subtilis, Japan]
BKE19650 ([gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
BKK19650 ([gene|0BF97861B52722347C4BBE533E574CF548A8283E|pgpB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTTAACCACCTCAATT, downstream forward: _UP4_TAGAGAGAGGAAAACACAGC
FLAG-tag construct
GP3675 (''spc'', based on [wiki|pGP1331]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2024-12-28 06:56:33
Author of last update: Rica