aprE
168
major extracellular alkaline protease
locus
BSU_10300
Molecular weight
39.37 kDa
pI
9.34
function
protein degradation
product
extracellular alkaline serine protease (subtilisin E)
essential
no
synonyms
aprE, sprE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,104,423 1,105,568
Phenotypes of a mutant
impaired biofilm architecture [pubmed|38922753]
The protein
Catalyzed reaction/ biological activity
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides(according to UniProt)
Protein family
[wiki|peptidase S8 family] (according to UniProt)
[wiki|Domains]
Inhibitor I9 domain (aa 38-109) (according to UniProt)
[wiki|Peptidase S8 domain] (aa 111-380) (according to UniProt)
Structure
[PDB|1SBC]
[AF|P04189]
[wiki|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
Operons
genes
[gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]
description
[pubmed|22383849]
regulation
repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR] [Pubmed|1898931]
induced in the presence of external [protein|60D6EB02923D9ADE88F61A8CBBD882BA8BDD457E|comX] [pubmed|29449835]
regulatory mechanism
[protein|5A6FBAE6553343092862CB79E150F934978C32A9|sinR]: repression, [Pubmed|1898931], in [regulon|protein:5A6FBAE6553343092862CB79E150F934978C32A9|sinR regulon]
[protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]: repression, [Pubmed|1906467], in [regulon|protein:F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC regulon]
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|2504584], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
[protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P, [Pubmed|12950930,15598897,2447063], in [regulon|protein:D343D2096664A972026D911E7A17A35C7B1CD1C9|degU regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|26728191], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2447063], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
additional information
in a triple ''[wiki|abrB] [wiki|codY] [wiki|scoC]'' mutant, expression occurs during exponential growth [Pubmed|26728191]
additional information
expression is increased in a [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] mutant due to the presence of increased levels of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P [pubmed|28800172]
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
BKE10300 ([gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
BKK10300 ([gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
Antibody
**
References
Reviews
Original Publications
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Time of last update: 2025-02-26 16:45:04
Author of last update: Jstuelk