tpi
168
triose phosphate isomerase, glycolytic/ gluconeogenic enzyme
locus
BSU_33920
Molecular weight
26.88 kDa
pI
4.79
function
enzyme in glycolysis/ gluconeogenesis
product
triosephosphate isomerase
essential
no
ec
5.3.1.1
synonyms
tpi, tpiA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0149 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,479,405 3,480,166
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth [Pubmed|28189581]
essential [Pubmed|12682299], non-essential according to [Pubmed|23420519]
poor growth [pubmed|28189581]
poorly transformable [pubmed|28189581]
The protein
Catalyzed reaction/ biological activity
D-[metabolite|glyceraldehyde-3-P] --> [metabolite|dihydroxyacetone-P] (according to UniProt)
Protein family
triosephosphate isomerase family (single member,according to UniProt)
Structure
[PDB|1BTM] (complex with [metabolite|2-phosphoglycolate], Geobacillus stearothermophilus)[http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=48255 NCBI]
[AF|P27876]
Modification
phosphorylation on Ser-213 [Pubmed|17218307]
Effectors of protein activity
inhibited by [metabolite|2-phosphoglycolate] (in ''B. stearothermophilus'') [Pubmed|8580851]
[wiki|Localization]
cytoplasm (homogeneously distributed throughout the cell) [Pubmed|24825009,16479537]
Additional information
extensive information on the structure and enzymatic properties of Tpi can be found at [http://www.proteopedia.org/wiki/index.php/Triose_Phosphate_Isomerase Proteopedia]
belongs to the 100 [http://subtiwiki.uni-goettingen.de/wiki//index.php/Most_abundant_proteins most abundant proteins] [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
regulation
expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] [Pubmed|11489127]
the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]: repression, [Pubmed|11489127], in [regulon|protein:ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11489127], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
genes
[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
description
[Pubmed|11489127]
Biological materials
Mutant
GP700 (cat), available in [wiki|Jörg Stülke]'s lab, [Pubmed|23420519]
BKK33920 ([gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33920 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGTTCCACTTCCTTAT, downstream forward: _UP4_GTTCAATTATTGGAGGAAGG
Expression vectors
pGP394 (N-terminal His-tag, in [wiki|pWH844]), available in [wiki|Jörg Stülke]'s lab
pGP89 (N-terminal Strep-tag, for [wiki|SPINE], expression in B. subtilis), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
pGP1511 (expression in ''B. subtilis'', in [wiki|pBQ200]), available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab, [pubmed|19193632]
References
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Time of last update: 2024-10-09 04:48:30
Author of last update: Jstuelk