yloS
168
thiamine pyrophosphokinase
locus
BSU_15800
Molecular weight
23.95 kDa
pI
5.22
function
thiamine salvage
product
thiamine pyrophosphokinase
essential
no
ec
2.7.6.2
synonyms
yloS, thiN
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1564 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,654,730 1,655,374
The protein
Catalyzed reaction/ biological activity
ATP + thiamine --> AMP + H+ + thiamine diphosphate (according to UniProt)
Protein family
thiamine pyrophosphokinase family (single member, according to UniProt)
Structure
[PDB|3LM8]
[AF|O34664]
Expression and Regulation
Operons
genes
[gene|84447F9A6644EA8A7593BB99B2B69D4377E670E2|prpC]-[gene|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|prkC]-[gene|519D4694B2895EE6F0D38D918F52F27734506848|cpgA]-[gene|8103B8539338B77CF51B16B7FA76783ED2319F13|rpeA]-[gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B136 (yloS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1135 NBRP B. subtilis, Japan]
BKE15800 ([gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT, downstream forward: _UP4_TAAGCATGTGCTTTCATTCA
BKK15800 ([gene|0ACE023DCD6A6BB5A1827D1D997BDFAC3B168C51|yloS]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15800 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT, downstream forward: _UP4_TAAGCATGTGCTTTCATTCA
References
Reviews
Original Publications
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Time of last update: 2025-04-03 21:50:40
Author of last update: Melvin.boenninger