pheS
168
phenylalanyl-tRNA synthetase (alpha subunit), universally conserved protein
locus
BSU_28640
Molecular weight
38.52 kDa
pI
5.08
function
translation
product
phenylalanyl-tRNA synthetase (alpha subunit)
essential
yes
ec
6.1.1.20
synonyms
pheS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0016 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,929,438 2,930,472
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + L-phenylalanine + tRNAPhe --> AMP + diphosphate + H+ + L-phenylalanyl-tRNAPhe (according to UniProt)
Protein family
[wiki|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)
Structure
[PDB|1PYS] (the [protein|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[protein|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT] complex from ''Thermus thermophilus hb8 mutant'', 37% identity, 56% similarity) [Pubmed|7664121]
[AF|P17921]
[wiki|Localization]
cytoplasm (according to Swiss-Prot)
Additional information
belongs to the 100 most abundant proteins [PubMed|15378759]
Expression and Regulation
Operons
genes
[gene|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[gene|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT]
description
[Pubmed|2127701]
regulation
induced by phenylalanine limitation ([wiki|T-box]) [Pubmed|19258532]
the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
regulatory mechanism
T-box: RNA switch, via tRNA controlled [wiki|RNA switch], in [regulon|other_regulator:T-box|T-box]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2127701], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
References
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Time of last update: 2025-02-10 01:18:24
Author of last update: Jstuelk