yxkO
168
ADP/ATP-dependent NAD(P)H-hydrate dehydratase, general stress protein, survival of ethanol stress
locus
BSU_38720
Molecular weight
29.72 kDa
pI
5.49
function
survival of ethanol stress, repair of hydrated NAD(P)H
product
ADP/ATP-dependent NAD(P)H-hydrate dehydratase
essential
no
synonyms
yxkO
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0063 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
3,972,448 3,973,278
The protein
Catalyzed reaction/ biological activity
(6S)-NADHX + ADP --> AMP + H+ + NADH + phosphate (according to UniProt)
(6S)-NADPHX + ADP --> AMP + H+ + NADPH + phosphate (according to UniProt)
Protein family
NnrD/CARKD family (single member, according to UniProt)
[wiki|Domains]
YjeF C-terminal domain (aa 7-275) (according to UniProt)
Structure
[PDB|1KYH] [pubmed|12457846]
[AF|P94368]
Expression and Regulation
Operons
genes
[gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]
description
[Pubmed|10746760]
regulation
induced by stress ([protein|search|SigB]) [Pubmed|15805528]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
Biological materials
Mutant
MGNA-B749 (yxkO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1748 NBRP B. subtilis, Japan]
BKE38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE38720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG
BKK38720 ([gene|0A9C77804C09CD5E7D1D5D274E01B5B14920A1A9|yxkO]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK38720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCTATCCCTCCTCTGC, downstream forward: _UP4_TTTGAATGATAAAAAAGCTG
References
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Time of last update: 2024-10-06 19:12:34
Author of last update: Melvin.boenninger