miaA
168
tRNA isopentenylpyrophosphate transferase
locus
BSU_17330
Molecular weight
35.52 kDa
pI
6
function
tRNA modification
product
tRNA isopentenylpyrophosphate transferase
essential
no
ec
2.5.1.8
synonyms
miaA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,866,389 1,867,333
Phenotypes of a mutant
inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
adenosine37 in tRNA + dimethylallyl diphosphate --> diphosphate + N6-dimethylallyladenosine37 in tRNA (according to UniProt)
Protein family
IPP transferase family (single member, according to UniProt)
Structure
[PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
[AF|O31795]
Expression and Regulation
Operons
genes
[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
genes
[gene|61B511C49912F1380AEE18AD300255A435F56F94|gerY]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation ] in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]) [Pubmed|39530705,22383849]
sigma factors
[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [pubmed|39530705], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]
Biological materials
Mutant
BKE17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT, downstream forward: _UP4_TAATCGAAACTGTATGATAT
BKK17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT, downstream forward: _UP4_TAATCGAAACTGTATGATAT
References
The corresponding gene/ protein in other bacteria
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Time of last update: 2025-04-04 14:53:06
Author of last update: Jstuelk