miaA

miaA
168

tRNA isopentenylpyrophosphate transferase

locus
BSU_17330
Molecular weight
35.52 kDa
pI
6
Protein length
Gene length
function
tRNA modification
product
tRNA isopentenylpyrophosphate transferase
essential
no
ec
2.5.1.8
synonyms
miaA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,866,389  1,867,333
Phenotypes of a mutant
inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
adenosine37 in tRNA + dimethylallyl diphosphate --> diphosphate + N6-dimethylallyladenosine37 in tRNA (according to UniProt)
Protein family
IPP transferase family (single member, according to UniProt)
Structure
[PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
[AF|O31795]
Expression and Regulation
Operons
genes
[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
Open in new tab

[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2024-09-28 05:23:09

Jstuelk

104

618ff8dc028666b7102a8ac70fd2e0e7e7cee80a

30921149A989DB9C49A175051521F780FA3F893A

genes
[gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation ][Pubmed|22383849]
Open in new tab

[gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2024-09-15 01:01:23

Jstuelk

105

4c22fbfe04a5390fcbae1a84c6631b5b7d9cc3d4

414C28CC98280BCBBDFD5DED7555018FF98C974C

Biological materials
Mutant
BKE17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
BKK17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
References
The corresponding gene/ protein in other bacteria
11160115,3045085,9294434,26735940,35212379

09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D

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Time of last update: 2024-10-04 16:19:21

Author of last update: Jstuelk