miaA

miaA
168

tRNA isopentenylpyrophosphate transferase

locus
BSU_17330
Molecular weight
35.52 kDa
pI
6
Protein length
314 aaSequenceBlast
Gene length
945 bpSequenceBlast
function
tRNA modification
product
tRNA isopentenylpyrophosphate transferase
essential
no
ec
2.5.1.8
synonyms
miaA
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0324 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,866,389  1,867,333
Phenotypes of a mutant
inactivation of ''[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]'' reduces sporulation efficiency to 16% that of wild type cells [Pubmed|26735940]
The protein
Catalyzed reaction/ biological activity
adenosine37 in tRNA + dimethylallyl diphosphate --> diphosphate + N6-dimethylallyladenosine37 in tRNA (according to UniProt)
Protein family
IPP transferase family (single member, according to UniProt)
Structure
[PDB|2QGN] (from ''Bacillus halodurans'', 57% identity)
[AF|O31795]
Expression and Regulation
Operons
genes
[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
Open in new tab

[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2024-09-28 05:23:09

Jstuelk

104

618ff8dc028666b7102a8ac70fd2e0e7e7cee80a

30921149A989DB9C49A175051521F780FA3F893A

genes
[gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]-[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]
description
[pubmed|22383849]
regulation
expressed during [wiki|sporulation ][Pubmed|22383849]
Open in new tab

[gene|61B511C49912F1380AEE18AD300255A435F56F94|ymaF]→[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

2024-09-15 01:01:23

Jstuelk

105

4c22fbfe04a5390fcbae1a84c6631b5b7d9cc3d4

414C28CC98280BCBBDFD5DED7555018FF98C974C

Biological materials
Mutant
BKE17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
BKK17330 ([gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17330 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATATGTATCACTCTCTTT,  downstream forward: _UP4_TAATCGAAACTGTATGATAT
References
The corresponding gene/ protein in other bacteria
11160115,3045085,9294434,26735940,35212379

09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D

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Time of last update: 2024-10-04 16:19:21

Author of last update: Jstuelk