yraA
168
general stress protein, degradation of damaged thiol-containing proteins, glyoxalase III-like enzyme
locus
BSU_27020
Molecular weight
18.37 kDa
pI
4.73
function
detoxification of methylglyoxal
product
glyoxalase III-like enzyme
essential
no
synonyms
yraA
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0693 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,757,492 2,758,001
The protein
Catalyzed reaction/ biological activity
methylglyoxal - D-lactate [Pubmed|24330391]
Protein family
[wiki|peptidase C56 family] (according to UniProt)
[wiki|Domains]
[wiki|PfpI endopeptidase domain] (aa 3-169) (according to UniProt)
Structure
[PDB|4Y0N] (from Staphylococcus aureus, 57% identity) [pubmed|26487697]
[AF|O06006]
Paralogous protein(s)
[protein|F88F0153CAFC1E51F2586F733AFDF22320642151|yfkM]
Expression and Regulation
Operons
genes
[gene|BEF882B7B846E3B8BF4C08FBF0DBB9F5A2CA2604|yrzP]-[gene|352544F3E79943E94E28A6EB5F4C543B529A02D1|adhA]-[gene|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|yraA]
description
[Pubmed|22383849]
genes
[gene|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|yraA]
description
NULL
regulation
''[protein|search|adhA]'': induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde ([protein|search|AdhR]) [Pubmed|19170879]
regulatory mechanism
[protein|B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR]: activation, [Pubmed|19170879], in [regulon|protein:B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR regulon]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|15805528], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]
[protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|17434969], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
genes
[gene|352544F3E79943E94E28A6EB5F4C543B529A02D1|adhA]-[gene|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|yraA]
description
[Pubmed|19170879]
regulation
''[protein|search|adhA]'': induced in the presence of the toxic carbonyls methylglyoxal and formaldehyde ([protein|search|AdhR]) [Pubmed|19170879]
regulatory mechanism
[protein|B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR]: activation, [Pubmed|19170879], in [regulon|protein:B24BA6ABCAF7325EC5F8B2655CA182FFC23395FD|adhR regulon]
Biological materials
Mutant
MGNA-A239 (yraA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/239 NBRP B. subtilis, Japan]
BKE27020 ([gene|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|yraA]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAACCCTCCTAATA, downstream forward: _UP4_TAACAAGAAGGCACAGACTG
BKK27020 ([gene|0960F1856A81237DBA6AF1FAF36172A63ECBE2D5|yraA]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAACCCTCCTAATA, downstream forward: _UP4_TAACAAGAAGGCACAGACTG
References
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Time of last update: 2024-10-14 22:21:55
Author of last update: Melvin.boenninger